RNA-seq of Lactobacillus johnsonii N6.2: Extracellular vesicle RNA re... - SRA

SRX18833440: RNA-seq of Lactobacillus johnsonii N6.2: Extracellular vesicle RNA replicate 1
1 ILLUMINA (Illumina NovaSeq 6000) run: 8.1M spots, 2.4G bases, 699.7Mb downloads

Design: Library construction and sequencing was performed by Novogene, (Novogene Co., Davis, CA, USA) as follows. RNA purity was checked using the NanoPhotometer spectrophotometer (IMPLEN, CA, USA), and RNA integrity and quantitation were assessed using the RNA Nano 6000 Assay Kit of the Bioanalyzer 2100 system (Agilent Technologies, CA, USA). A total amount of 1 g RNA per sample was used as input material for the RNA sample preparations. Sequencing libraries were generated using NEBNext UltraTM RNA Library Prep Kit for Illumina (NEB, USA) following manufacturers recommendations and index codes were added to attribute sequences to each sample. After cluster generation, the library preparations were sequenced on an Illumina platform and paired-end reads were generated. Raw data (raw reads) of FASTQ format were first processed through fastp. The reference genome (NCBI NC_022909.1) and gene model annotation files were downloaded from NCBI.

Submitted by: University of Florida

Study: Internalization of extracellular vesicles from Lactobacillus johnsonii N6.2 elicit an RNA sensory response in human pancreatic cell lines.

PRJNA915103 • SRP414532 • All experiments • All runs

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Global transcriptome analysis of RNA extracted from Lactobacillus johnsonii N6.2 and its purified extracellular vesicle to evaluate the impact of the RNA cargo in the EV on the upregulation of the cellular response of Beta-lox5 human pancreatic cells and identify potential selectivity in EV RNA transcript enrichment.

Sample: Lactobacillus johnsonii N6.2 extracellular vesicle replicate 1

SAMN32377071 • SRS16264636 • All experiments • All runs

Organism: Lactobacillus johnsonii

Library:

Name: EV_01

Instrument: Illumina NovaSeq 6000

Strategy: RNA-Seq

Source: TRANSCRIPTOMIC

Selection: RANDOM

Layout: PAIRED

Runs: 1 run, 8.1M spots, 2.4G bases, 699.7Mb

Run	# of Spots	# of Bases	Size	Published
SRR22874886	8,058,142	2.4G	699.7Mb	2024-01-31

ID:: 25924630

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